Antinutrients Testing

Antinutrients Testing

Precise control of antinutrients in food can help to enhance the core competitiveness of your products. With professional and rich experience in food testing, Aprofood provides you with one-stop antinutrient analysis solutions to help product compliance, nutritional upgrading, and brand trust building.

Antinutrients in Food Products

Antinutritional factors are natural defense mechanisms that have evolved during plant evolution and are therefore widely present in plant-based foods. These compounds can interfere with the absorption, metabolism, or utilization of essential nutrients. Many traditional food processing methods, such as heating and fermentation, can effectively reduce or destroy antinutrients. For example, soaking beans for 12 hours can reduce phytic acid content by 30–50%, boiling beans for 30 minutes can inactivate over 90% of lectins, roasting nuts can lower tannin levels, and fermenting foods like yogurt and sauerkraut can break down phytic acid and protease inhibitors, thereby improving nutrient bioavailability.

Fig. 1 Anti-nutritional factors in food and their health impacts. Fig 1. ANFs in food and their health effects. (Salim R, et al., 2023)

Our Service

Aprofood is committed to high-quality testing services using advanced analytical technologies such as HPLC-MS, ELISA, and molecular blotting techniques. We provide antinutrient testing programs covering the whole category (plant-based foods, functional foods, herbal extracts, etc.) and the whole scenario (raw material testing, finished product compliance), accurately quantifying 20+ antinutritional factors, which mainly cover and are not limited to the following:

Test Item Sample Type Test Method
Phytic Acid/Phytates Grains, legumes, nuts, seeds, plant-based beverages, frozen foods (frozen peas) HPLC-MS/ICP-OES
Oxalic Acid/Oxalates Vegetables (spinach, beets), fruits (grapes, figs), chocolates, beverages LC-MS/TOC Analyzer
Lectins Legumes, cereal products, plant-based meats (vegan chicken, burgers) ELISA/Biosensor
Tannins Tea (green, black), wine, walnuts, acorns, candies Folin-Ciocalteu Method
Glucosinolates Cruciferous vegetables, sprouts, condiments (mustard, horseradish) HPLC-UV/ELISA
Isoflavones Soy products (tofu, soy milk), soy protein isolates, herbal supplements HPLC-QTOF-MS
Saponins Herbs (ginseng, notoginseng), beverages (herbal tea), legumes (chickpeas) HPLC-UV/ELISA
Trypsin Inhibitor Soy, oilseed meals, animal feed, fermented foods (natto, miso), pet food ELISA/Turbidimetry
Gossypol Cottonseed products, herbal extracts, feed additives HPLC-UV/GC-MS
Protease Inhibitors Cereals (wheat, barley), legumes, functional foods Fluorometric Assay
Lipase Inhibitors Grains (wheat, oats), whole wheat flour, functional fat substitutes Enzymatic Activity Assay
Amylase Inhibitors Tubers (potatoes, yams), legumes (mung beans), supplements Iodine-Amylose Assay
Gluten Cereal-based products (bread, pasta), seasonings (soy sauce, miso) R5 ELISA

Frequently Asked Questions (FAQs)

Q1: What should be noted when sending samples?

Q2: What is the minimum detection limit?

Q3: How is the detection accuracy verified?

Q4: What does the test report include?

Aprofood responds quickly to customers with customized testing solutions to help them avoid quality risks associated with antinutrients, enhance product competitiveness in the marketplace, and ensure compliance with nutrition labeling and regulatory requirements. Whether you are a manufacturing plant, a food brand, or an R&D organization, contact us to learn more about our antinutrient services.

Reference:

  1. Salim R.; et al. A review on anti-nutritional factors: Unraveling the natural gateways to human health[J]. Frontiers in Nutrition. 2023, 10: 1215873.
For Research Use Only.
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