Probiotics Development Service

Probiotics Development Service

Probiotics are now emerging as an important category in food supplements and dominating the functional food ingredients market. Aprofood is constantly engaged in basic scientific research, working on selecting and cultivating specific strains to provide you with probiotic development services.

Sources and Classification of Probiotics

Probiotic strains have been derived from various sources, such as dairy products, plants, soil, fermented substrates, the gastrointestinal tract of humans and other animal species. Commercial probiotic products commonly include:

  • The yeast Saccharomyces boulardii, which has been shown to prevent diarrhea.
  • Some of the normal GI inhabitants, such as the genera Lactobacillus, Bifidobacterium, or Enterococcus.
  • Some bacteria are used as “starters” in the manufacturing of dairy products, which normally do not inhabit the human intestine, including L. bulgaricus, Streptococcus thermophilus, and species of Leuconostoc and Lactococcus.

Action mechanisms of probioticsFig 1. Action mechanisms of probiotics. (You S, et al.,2022)

Benefits of Probiotics

Probiotics are live microorganisms that occur naturally in certain foods or can be added during food production. Consuming probiotics can provide various benefits to the host, such as:

  • Improving intestinal health by the regulation of microbiota.
  • Stimulating and regulating the immune system.
  • Synthesizing and increasing the bioavailability of nutrients.
  • Reducing the symptoms and risk of certain diseases.

Our Service

With advanced microbiological technologies and laboratory equipment, Aprofood helps our clients identify, isolate and characterize probiotic candidate strains from various sources.

Traditional Probiotics Development Service

This includes isolating unique probiotic strains of existing species such as Lactobacillus, Bifidobacterium, or Enterococcus from traditional fermented dairy products and the fecal microbiome.

Process of Traditional Probiotics Development

Sample Collection

Collect samples from various sources mentioned above. We adhere to ethical considerations, ensure appropriate collection methods and sampling techniques to avoid contamination. Prepare the sample solution for further analysis by diluting the collected sample with a suitable diluent or buffer.

Isolation & Selection

Isolation of individual microorganisms by applying selective media and appropriate isolation techniques. Select colonies based on their morphology to find potential probiotic strains.


Transfer selected colonies onto fresh agar plates using aseptic techniques to isolate individual colonies, and the subculture process is repeated as needed to obtain pure cultures of the strains.


Ensuring the accurate identification and classification of strains and tracking of specific strains throughout the development process (traceability). We have expertise in molecular biology methods and mass spectrometry methods to accurately identify strains with potential probiotic properties.


Further characterization of selected strains to assess their compatibility with food matrices, stability under processing conditions and potential health benefits.

The Next-generation Probiotic Development Service

We have mastered next-generation sequencing technologies, bioinformatics tools, and advanced gut microbial culture technologies to help our customers isolate and identify new microorganisms from new sources such as the gut microbiota and modify them as candidate strains for next-generation probiotics.

Developing a mature food probiotic product requires characterization of its safety and performance, verification of its bioactive function, as well as investigation of its formulation techniques for food application and its compatibility with food matrices. Here you can get a one-stop service for product development.

Why Choose Us?

Wide Range of Ingredients

We are a one-stop shop for all types of food ingredients.

Innovative Program

We have a systematic development program to meet innovation needs.

Customized Formulation Portfolio

We develop customized additive combinations to optimize value for all types of foods and beverages.

Quality Assurance

We have the highest standards of production and quality testing procedures.

Aprofood is a reliable partner for you, we provide you with high-quality food ingredients and help you find the optimal solution for food development and innovation. Please contact us for more information on developing your business.


  1. You, S.; et al. The promotion mechanism of prebiotics for probiotics: A review. Front. Nutr. 2022, (9):1000517.
For Research Use Only.
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